Above Oxeye Daisy (Leucanthemum vulgare) at various magnifications including an aphid living inside the flower that was extracted and viewed by scanning electron microscopy showing the insect's compound eye and a single bacterium on the surface of the eye. These photos show the range of magnifications I offer.
Above is a wide variety of substances including wood, caffeine, bone, citric acid and starch grains viewed with a polarizing microscope.
If you require high magnification photographs for industry, medicine, forensic, education or personal use, including fiber optic, minerals, microchips, plankton, insects, Diatoms, Foraminifera, fossils, sand grains, coins, postage stamps, textiles, starch grains, botanical subjects, hair and other items contact me. I provide high quality images using specialized macrophotography and microscope equipment. By focus stacking multiple images I can increase the depth of field several fold. I currently own nine research quality microscopes providing specialized illumination techniques:
Phase contrast - for viewing living cells
Differential Interference Contrast - (DIC) viewing living cells and small organisms.
Hoffman modulation - living cells, black and white only provides 3 D appearance
Polarization - for birefringent specimens, hair, wood, crystals etc
Fluorescence - plants and other biological samples
Rheinberg lighting - any specimens
Metallurgical microscopy - I can photograph opaque specimens and surfaces, metals etc
Darkfield illumination - aquatic organisms
Brightfield illumination - stained slides and some aquatic organisms
Comparison of a ciliate Paramecium bursaria Left: bright field microscopy and Right: Differential interference microscopy 400X.
My light microscopes can resolve objects as small as 0.2 microns (0.0002 mm) and components to about 20 nm with the scanning electron microscope. I provide image processing and optimization of the images. I can provide digital images on a CD\DVD, USB stick or portable hard drive. I have special tools for micromanipulation and can also prepare permanent microscope slides if required. I also provide video taken through the microscope and time lapse movies - see example of water bear on the bottom of my article. If you would like something photographed for artistic reasons, decorative purposes, post cards, posters or for educational purposes let me know.
Citric acid crystals by Polarization Microscopy 100X
I am member of the University of Calgary Microscopy Group, an adjunct assistant professor in the Dept. of Cell Biology and I have access to the electron microscopes. I am a trained scientist in the fields of Cell Biology, Neurobiology and Biochemistry with a passion for microscopy with over 45 years experience. I am also a professional Nature photographer and teacher and taught web design and photography at SAIT for over 20 years.
Darkfield macrophotograph of a Trout Alevin 5X
Radiolarians single cell plankton live in the ocean and form silica shells 400X Darkfield microscopy
Hexagonal symmetry of a snowflake 20X
Section through white Pine 400X Polarizing microscopy and full wave plate.
Leaf Section 100X Brightfield microscopy - panorama of images stitched together
My private microscopy lab showing some of my microscopes.
Left: Nikon Optiphot Polarizing\Fluorescence microscope Right: Zeiss Axioscope with DIC, Phase contrast, Polarizing and Darkfield illumination.
Phacodinium metchnikoffi a ciliate found in moss - Differential interference microscopy (DIC) 400X
Potato starch grains Polarized light with full wave plate 200X.
Above Citric Acid crystals in Polarized light - Abstract 100X
Wild Stereo microscope with a Canon 5D Mark II and electronic flash, ring-light and side lighting, capable of 1-50X magnification.
Canadian Penny photographed using the Wild Stereomicroscope.
Custom macro stand with Canon 80D and Canon MP 65-E lens with focus rail and high speed flash capable of 0.5 to 15X magnification.
Painted Lady butterfly provided by an Alberta entomologist - 36X Focus stacked and photographed with the Canon camera and MP 65-E lens shown above.
Left - Right: Olympus metallurgical microscope, Olympus SZM III Stereomicroscope, Olympus E microscope with Phase contrast, Olympus IMT inverted microscope with Phase contrast.
Freshwater Diatoms photographed with the Olympus E microscope and Darkfield illumination. 400X
Scanning Electron Microscopy
Isolated neuron from the buccal ganglion of a Fresh water snail, Helisoma trivolvis, showing lipid vesicles below the plasma membrane by Scanning electron microscopy. False coloured 1000X
Scanning electron micrographs of single isolated neurons in culture for science publication.
Aphid low magnification using a Scanning electron microscope (SEM) 20X False coloured.
Head of an Aphid SEM 200X - false coloured in Photoshop
Charcoal showing individual cells by SEM
Scanning electron microscope at the University of Calgary - the microscope is available at an hourly rate.
Training and Equipment Rentals
I offer training in all aspects of macrophotography, and photomicrography for $60\hr or $299 per day in my studio. After receiving some training you can rent the use of my equipment for $40\hr in my studio and then take your digital images on a USB or portable hard drive and the images belong to you. I provide private training to anyone interested including photo editing in Photoshop and Focus stacking - contact me for more information or to book a training session. I can also recommend equipment, stereomicroscopes and light microscopes if you are considering purchasing one. Fortunately there is a Zeiss dealer across the street from me specializing in stereo microscopes for geology - visit his web site Quality Microscopes Ltd and for new comers to microscopy I recommend light microscopes from AM Microscope which offer good quality for lower cost than the name brands. If you are knowledgeable about microscopes you can purchase used name Brand microscopes on E-bay for significant savings though they may need refurbishing. For top quality new microscopes I recommend: Zeiss, Nikon, Olympus, Leica and Wild but their prices are significantly higher. I can also refurbish some microscopes - if interested in my repair services contact me.
Dr. Robert Berdan working on his Zeiss Axioscope. Robert has a BSc in Zoology, MSc in Cell Biology, PhD in Cell biology, Postdoctoral studies in neurobiology at University of Calgary and he worked as a Heritage research scientist at the University of Alberta for 5 years. Robert started his own business Science & Art over two decades ago. His passion for photomicrography lead to a career in research and he now focuses on using his knowledge of microscopy to offer his photography services. His photographs have been published in magazines and science papers and he has received numerous awards. He uses Nikon and Canon Digital cameras to capture images.
To book services or inquire about training call 403 247-2457 or send an email to email@example.com. Robert is available most weekdays 9-5, some evenings and weekends.
To see more photomicrographs please visit my galleries and articles on my other web site www.canadiannaturephotographer.com. You can purchase of any of my digital images for personal or commercial use - see my prices.
If you have a budding scientist in your family I would be happy to provide an hour or two of free microscopy instruction in my studio for anyone between the ages of 12-17 under the supervision of a parent or guardian. I am happy to answer any questions you might have regarding microscopy and for a small honorarium I would be willing to come to your school or organization in or around Calgary to provide a presentation on microscopy and photomicrography. Teachers interested in learning more about microscopy are also welcome to visit me by appointment in my studio in Calgary, NW. My interest in microscopy began in Grade 6 and lead to a career in research and I would love to encourage others to take up microscopy as a hobby or a profession. The microworld is all around us waiting to be discovered - you can find interesting things in your backyard, nearby pond or in the soil. I have found interesting micro-organisms in rainwater from my evestrough and living in lichen and moss in my backyard.
Article by Dr. Robert Berdan - Photomicrography a Marriage of Science & Art - PDF